1·Objective To construct a recombinant plasmid containing rhoptry protein 2 (ROP2) gene of Toxoplasma gondii .
目的构建弓形虫棒状体蛋白2 （ROP2 ）基因重组质粒。
2·A plasmid containing the gene which is autonomously replicable in Enterobacteriaceae bacteria is also described.
本发明还描述含有该基因的质粒，其在肠杆菌科细菌中可自主复制。
3·The atrazine-degrading genetically engineered microorganism(GEM) was labeled by transforming a plasmid containing green fluorescent protein(GFP) gene.
通过转化绿色荧光蛋白基因质粒，对阿特拉津降解基因工程菌进行标记。
4·To construct a recombinant plasmid containing protein transduction domain (PTD) and brain derived neurotrophic factor (BDNF) fusion gene and express in e.
构建含蛋白转导结构域(PTD)与脑源性神经营养因子(BDNF)融合基因的质粒，并在大肠肝菌中表达。
5·These results indicate that injection of the recombinant plasmid containing hLYZ cDNA can be used as an alternative strategy for treating and preventing dairy cow mastitis.
因此，该基因药物可以作为替代抗菌素等常规药物用于防治奶牛乳腺炎的新型药物加以开发应用。
6·Objective To construct a recombinant plasmid containing P30 gene of Toxoplasma gondii and obtain recombinant protein Methods P30 gene fragment was amplified from genomic DNA of T.
目的构建表达P30蛋白抗原表位的重组质粒及工程菌，获得纯化的P30蛋白抗原表位，用于检测弓形虫特异性抗体。
7·Objective:To construct a recombinant plasmid containing rhoptry protein 2(ROP2)gene of Toxoplasma gondii and express it in E. coli for selection of diagnostic antigen and vaccine candidate.
目的：构建弓形虫棒状体蛋白（ROP2 ）基因重组质粒并在大肠杆菌中表达，用于筛选弓形虫新的诊断抗原和疫苗分子。
8·Methods: The gastric carcinoma cells had been successfully transfected with plasmid containing the C' distal end antisense sequence of glutaminase gene and called SGC-7901GA in previous researches.
方法：本课题前期阶段已将含谷氨酰胺酶基因C末端反义序列的质粒转染入胃癌细胞并命名为SGC-7901~（GA）。
9·Objective to clone and construct the plasmid containing human thyroid stimulating hormone receptor (TSHR) gene ectodomain, and then identify the immunoreactivity of the purified recombinant protein.
目的克隆人促甲状腺激素受体胞外段基因，构建重组真核表达质粒，获得具有免疫学活性的纯化重组蛋白。
10·In vitro DNA replication system derived from eukaryotes is capable of replicating exogenous plasmid DNA containing SV40 origin of replication efficiently and accurately.
报道了含sv40复制起点的质粒dna在真核细胞抽提物中进行复制的DNA体外复制系统的建立。